奥林巴斯BX53报价和功能

　　传统单点扫描与转盘式扫描的区别：

工程师 张帆 13810005848

    转盘式是多点同步扫描，转盘上分布有20000个螺旋排列的针孔，而激光光源覆盖约2000个针孔的范围(即扫描区域)，借助转盘的转动，实现对样品的完整扫描。相当于2000个激光点同步照射样品，同步激发，CCD上同步采集，所以扫描速度非常的快。转盘式是目前活细胞共聚焦非常先进的技术，最大的特点是速度快，灵敏度高，光漂白和光毒性水平低，最适合活细胞、快速、6D扫描研究。

    　　Yokogawa双转盘式共聚焦扫描技术

　　Yokogawa的CV1000转盘式共聚焦扫描成像系统是定位于长时间、多时相、6D活细胞成像的一体化共聚焦系统，它整合了显微镜，共聚焦光路系统，以及活细胞工作站等诸多模块于一体，不需暗室操作并且集成了温度、湿度控制等繁琐过程。整套安装简易，操作简单，使用方便，并且厂家携代理商提供完善的售后服务。

　　CV1000使用的双转盘共聚焦扫描头，是活细胞成像的金标准，最大限度地降低了光毒性和光漂白。CV1000是目前唯一的能长时间对胚胎干细胞做3D成像，然后被观察的胚胎干细胞能顺利发育为成体小鼠的系统。该系统的精确的温度控制系统(精确度可达0.1℃)，使得干细胞，胚胎，以及其他细胞可在稳定的温度环境中生长，精确地完成您的实验操作。为活细胞的观察在技术水平上提供了双保险，使得CV1000更适合于非常适合于精确度要求极高的生命过程的观察，如发育生物学，干细胞研究等领域的长时高速6D成像，尤其适合于干细胞增殖和分化，iPS细胞成像，胚胎发育，转基因细胞实时观察等领域。

　　Software for Image Analysis

　　Voxx : Written by Jeff Clendenon and Jason Byars

　　A voxel-based (not surface-based) 3D imaging program capable of near real-time rendering of large microscopy data sets on inexpensive personal computers has been developed in the Indiana Center for Biological Microscopy. It was designed to use the low-cost (NVIDIA GeForce) 3D graphics processors found on video boards in many lab and office PCs, instead of the more expensive voxel processors or graphics workstations commonly used by others for voxel-based rendering. The program currently runs on Windows 98/NT/2000, with Apple MacOS and Linux ports underway.

　　Confocal Assistant (ver 4.02): Written by Todd Clark Brelje

　　A very neat and powerful program that reads Bio-Rad PIC images. It allows users to make format conversion, projection, movies, color encoding, montages, etc. Those who use Metamorph (Universal Imaging) will still find Confocal Assistant useful. It is a lot faster than Metamorph when you make projections. Best of all, it''s free!

　　ImageJ : From the NIH

　　A free image processing tool from the NIH. It has an extesive library of plugins.

　　Zeiss LSMoffline browser R3.5: From Carl Zeiss, Inc.

　　You need it to convert your LSM images to Tiff format. If you have problem installing and have DAO related error messages, you may need the dao.zip fil

　　LCSlite：From Leica

　　ImageTool

　　Confocal Assistant

　　Version 4.02

　　An amazing little program written by Todd Clark Brelje for reviewing and manipulating BioRad .pic image stacks

　　Zeiss LSM Image Examiner

　　Version 3.5

　　From Carl Zeiss, Inc. You will need it for browsing your Zeiss libraries away from the microscope.